Navegando por Assunto "Abelhas sem ferrão"
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Item Análise do sistema de criação da abelha Melipona scutellaris em Bonito - Pernambuco(2022-06-01) Costa, Jóselly Rodrigues da; Gomes, Renata Valéria Regis de Sousa; http://lattes.cnpq.br/8551019809231664; http://lattes.cnpq.br/6712138440975289This study aims to survey the breeders of the Northeastern uruçu bee (Melipona scutellaris) in the municipality of Bonito, demographically characterizing the area and the breeding system. Semi-structured and free interviews were carried out and 9 (nine) meliponaries were identified. The presence of beekeepers was verified in the regions demographically classified as Agreste, Brejo de Altitude and Zona da Mata, being the properties of the Rural type. Regarding the number of hives, 101 colonies of uruçu bee were observed, with an average of 11.2±12.1 for the 9 evaluated meliponaries, where only 01 creator contained the amount of 42 colonies and the others varied from 02 to 15. If the traditional uruçu bee breeders in Bonito-Pernambuco, most of them have a breeding system with a low technological level, making it necessary to take actions that lead them to perceive themselves as true guardians of this biodiversity, in order to conserve this genetic resource in a sustainable way. continued with self-sustainable management.Item Associação entre fungos e abelhas-sem-ferrão do semiárido de Pernambuco(2021-06-25) Silva, Aparecida Clébia da; Carvalho, Airton Torres; http://lattes.cnpq.br/5995654016063333; http://lattes.cnpq.br/3574387196330564Throughout the evolution of species, insects and fungi have developed important mutualistic interactions, in which both benefit. An interesting example is the bee of the genus Scaptotrigona spp. (Hymenoptera: Apidae, Meliponini), in which a species of fungus Zygosaccharomycessp. develop and produce important metabolites for the growth of immatures, being essential for the insect to complete its life cycle. In this context, the objective was to investigate the presence of filamentous fungi, associated with larval food, in brood cells with larvae at different stages of development in colonies of Scaptotrigona sp. tubiba group, in Caatinga areas of the state of Pernambuco. The collections were carried out in five colonies in 10 brood cells, being numbered from 1 to 10, in which 1 to 5 were collected in cells with developing larvae, from 6 to 7 only with larval food and egg and from 8 to 10 developed larvae. The samples were solubilized in sterile water and seeded on three culture media for isolation Sabouraud Agar (SAB), Potato Dextrose Agar (PDA) and Potato Dextrose Agar with an additional 15% of glucose (15% PDA). After five days of incubation at 28 ºC, 19 isolates were selected based on morphotypes. Amongst these, 3 were identified at the genus level, being a Fusarium, a Cladosporium and an Arcopilus, which was submitted to macro and micromorphological characterization for presenting characteristics similar to a species described in the literature. Subsequently, the growth capacity of Arcopilus sp. in four different conditions: pure larval feed, larval feed plus distilled water (1:1; v/v), Sabouraud broth and distilled water plus 1% glucose in wells of ELIZA plates. The greatest growth was observed in wells that were seeded with larval feed diluted in distilled water. The presence of Monascus sp. in larval food samples of Scaptotrigona sp. studied. The other isolates were stored for later identification of the species and it is expected that these fungi may actually be essential in the bee nests of this species.Item Atividade de voo da abelha moça branca (Frieseomelitta doederleini) no período de inverno na cidade de Recife - PE(2022-09-26) Côrte Real, Mirela Lima; Souza, Darclet Teresinha Malerbo de; http://lattes.cnpq.br/3266223126925865Bees have been gaining a great prominence in recent years, because people are increasingly looking for organic and sustainable products. Beekeeping, both stinging bees and melipona bees, fit these standards, and show themselves as an alternative breeding and source of income, especially for small producers. This work aims to verify the flight and collection activities of the white lady bee (Frieseomelitta doederleini.) in August and September, in the city of Recife, Pernambuco, metropolitan region. The present study was carried out from August 2022 to September 2022 in the meliponary of the Apiculture and Meliponiculture Sector, Department of Animal Science of the Universidade Federal Rural de Pernambuco, located in the neighborhood of Dois Irmãos, in Recife - PE. The boxes chosen for observation were randomly selected and observations were performed three days a month. The observations were made in the period from 09:00 am to 4:00 pm, for one minute each hour. In the observations, the number of bees that entered the colony carrying pollen and the number of bees that entered with no apparent cargo were counted, being counted as nectar or water. Temperature and relative humidity data were also recorded with a digital thermohygrometer installed in the meliponary. The movement of the bees was greater in the morning, a pattern that was repeated in all months. The tendency was greater collection of nectar and water, when compared to pollen collection. Thus, it was concluded that the bees (Frieseomelitta doederleini) prefer to collect both nectar and pollen in the morning, decreasing their frequency during the day in the months studied.Item Atividade de voo da abelha mosquito (Plebeia sp.) em meliponario urbano, na cidade de Recife - PE(2021-11) Farias, Lucas Rodrigues de; Souza, Darclet Teresinha Malerbo de; http://lattes.cnpq.br/3266223126925865; http://lattes.cnpq.br/3842757406890382The search for products of organic and sustainable origin has been growing over the years, and the raising of bees with both sting, beekeeping, as well as stingless, meliponic growers fit these standards, and shows itself as an alternative for breeding and a source of income , mainly to small producers. This work aims to verify the flight and collection activities of the Mosquito bee (Plebeia sp.) in different months of the year, in the city of Recife, Pernambuco, a region in the forest zone. The present study was carried out from September 2021 to November 2021, in the meliponary of the Beekeeping and Meliponic Growing Sector, Department of Animal Science of the Federal Rural University of Pernambuco, located in the neighborhood of Dois Irmãos, in Recife – PE. The boxes chosen for observation were randomly selected and observations were carried out three days a month. Observations were made from 8:00 am to 5:00 pm, for ten minutes every hour. In the observations, the number of bees that entered the colony carrying pollen and the number of bees that entered without apparent load were counted, being counted as nectar or water. Temperature and relative humidity data were also recorded, with a digital thermohygrometer installed in the meliponary. All data were statistically analyzed using the ASSISTAT program. To compare means, when necessary, the Tukey test was used at a 1% probability level. To analyze the frequency of insect visitation to flowers during the day, regression analysis by orthogonal polynomials was used, thus obtaining adequate equations for the observed patterns, under the conditions of the experiment. The temperature and humidity data were as expected for each month of the year in a place with a humid tropical climate, typical of the northeastern coast. The movement of bees was greater in the morning. The trend was towards greater nectar/water collection when compared to pollen collection. Thus, it is concluded that Plebeia sp. stingless bees prefer to collect both nectar and pollen in the morning, decreasing their frequency during the day, in studied months of the year.Item Atividade de voo da abelha uruçu nordestina (Melipona scutellaris) nas diferentes épocas do ano(2021-06-30) Andrade, Milena Oliveira de; Souza, Darclet Teresinha Malerbo de; http://lattes.cnpq.br/3266223126925865; http://lattes.cnpq.br/7490394190634816The search for products of organic and sustainable origin has been growing over the years, and the creation of bees with both sting, beekeeping and stingless, meliponiculturers fit these patterns, and is shown to be an alternative for breeding and source of income, especially for small producers. This study aimed to verify the flight and collection activities of the northeastern uruçu bee (Melipona scutellaris) at different times of the year, on the UFRPE campus, located in the city of Recife, Pernambuco, zona da mata region. This study was conducted from September 2020 to June 2021. The observations were made three days in the beginning of each season, from 8:00 a.m. to 5:00 p.m., for ten minutes each hour, where the number of bees that entered the colony carrying pollen and the number of bees that entered without apparent load were counted. The temperature and humidity data were as expected for each time of year in a place with a humid tropical climate, typical of the northeastern coast. The movement of bees was greater in the morning, a pattern that was repeated at all times of the year. Comparing each season, the trend was for greater nectar/water collection, when compared to pollen collection, with the exception of autumn, where there was a higher collection of pollen. Thus, it was concluded that stingless bees Melipona scutellaris prefer to collect both nectar and pollen in the morning, decreasing their frequency throughout the day, at all seasons of the year.Item Bactérias do mel de abelhas sem ferrão (apidae: meliponini) com potencial antagônico a microrganismos patogênicos de interesse para saúde humana(2021-12-13) Figueroa, Marcos Vinicius; Fernandes, Hélio de Melo; http://lattes.cnpq.br/6890648212766368; http://lattes.cnpq.br/0173708664534934Stingless bee colonies are a natural reservoir of microorganisms that may be present in honey, pollen and other microenvironments throughout the nest. Bees, like other social insects, have complex symbiotic interactions that, throughout evolution, provided an ecological interaction that helped preserve the hives, favouring the life of these insects and giving them a survival advantage. Several microorganisms associated with stingless bees, mainly sporulating bacteria of the Bacillus genus, produce substances that inhibit the growth of competing microorganisms that contaminate and deteriorate the food stored in the hives. In this context, this work aimed to isolate bacteria from stingless bees of the apidae: meliponini group to verify the capacity of the microbiota against pathogenic microorganisms of interest to human health. In the antagonism test, samples of honey from Melipona asilvai with 10-1 to 10-4 dilutions were used, which were then submitted to hyperthermia in a water bath at 80˚C. Ten sporogenic bacterial morphotypes were selected and submitted to an antagonism test, but only six inhibited the growth of pathogenic bacteria with an inhibition halo ranging from 1 to 3 mm. Honey samples from M. subnitida, scaptotrigona sp and Friosiomelitta stored for more than two years and from Melipona asilvai showed absence of yeasts, filamentous fungi and thermotolerant total coliforms. All honey samples showed total mesophilic aerobic bacteria, in concentrations ranging from 2.9 x 104 a 9.79 x 104 CFU/g of honey. Although honey has high concentrations of sugars that inhibit microbial growth, several bacteria are able to resist high osmotic pressure and survive in this substrate, making it a natural reservoir of microorganisms that end up being beneficial to the colony and serving as a barrier against contaminating microorganisms.Item Conhecimentos e emoções em relação às abelhas e outros animais por estudantes de escolas públicas da Zona da Mata Norte, Pernambuco, Brasil(2021-03-05) Santos, Rayane Karoline Silva dos; Alves, Ângelo Giuseppe Chaves; http://lattes.cnpq.br/9733905079544308; http://lattes.cnpq.br/9755859774741987Human beings have "an Sate emotional tendency to affiliate with other living beings". This affiliation would have evolved Sing biocultural evolution, which allowed human beings to evolve successfully. "Love" for nature is an important factor that drives lmmans to conserve nature. However, in contrast to positive effects (biophilia), there is biophobia, which consists of the predisposition of human beings to express negative effects, such as fear, aversion, ad other feelings.' These negative feelings are often intended for insects. The activity of stingless bee raising promotes the conservation of these insects, stimulating biophilic attitudes towards these animals. The school provides a broad place for discussions and approaches to topics related to nature conservation. Thus, it is believed that students who are related with meliponicultors present, in their speeches and in their school activities, knowledge about stingless bees and other biodiversity components. Therefore, the objective of this study was to investigate the knowledge and attitudes related to animal diversity with students in a public school, in a rural location, where several farmers who raise native bees live. The research was carried out in the municipalities of Timbaiba and Macaparana with students who lived in the locality of Sitio rmai, where several meliponicultors live. A free list with Ewe questions ("Which animals the Xixi forest has?'; "Which animals does your family raise?"; "Which animals do you like most?"; "Which animals do you like least?" and "Write about the Uruott bee') was applied. The first four questions were analyzed using Anthropac that generated salience indices, while the last one was analyzed using descriptive statistics. Through the results, it was possible to identify the animal category "snake" as the most prominent for the first question, while for the second one the category "dog" was the most relevant For the third question, which talk about positive feelings, the categories "cat" and "dog" were the most evident, showing how domestic animals are more easily perceived in a positive way. In contrast, the fourth question, which is about negative feelings, had the category "snake" highlighted again. For the fifth question, the students demonstrated different knowledge about the Unica bee, emphasizing the categories ecological-scientific, utility and management and behavior. Thus, it is possible to in that students present different knowledge about unicu bees and the local Lima, presenting more biophobic than biophilic relationships with the bees.Item Estudo químico e atividade sequestradora de radical livre da própolis de Trigona spinipes Smith (Arapuá)(2022-06-10) Souza, Hélter José Silva de; Silva, Tania Maria Sarmento da; http://lattes.cnpq.br/9185334632055347The stingless bee Trigona spinipes is popularly known as a primordial generalist species in the performance of ecosystem services, due to the speciesization of agri-cul-tural plants, as a dominant species in the interaction networks between plants and bees. Geopropolis, is a resin mixture collected from pro-resin resins, essential oils, pollen, wax, sugars and salivary secretions from bees. This work describes the chemical study of two samples of Trigona spinipes propolis collected in the Caatinga region of the Polo Petrolina-PE, in the areas that comprise the Vale de São Francis-co. The chemical study of the extracts and fractions was carried out by Ultra Perfor-mance Liquid Chromatography coupled with Diode Array detectors and Quadrupole Time-Time Quadruple Mass Spectrometer (UPLC-DAD-QTOF-MS/MS) and coupled Gas Chromatography a Mass Spectrometer (CG/MS). From the chemical study it was possible to observe the presence of substances from the classes of polyamines, fla-vonoids, terpenes, steroids and fatty acids. The two have similar chemical com-posi-tion. The biological study with total phenolics, flavonoids and the DPPH radical showed that propolis are rich sources of bioactive compounds with antioxidant po-tential. The pro-anti-inflammatory action presents protection against bacteria, virus, fungi and anti-inflammatory activities.Item Fatores ambientais e oviposição: análise do comportamento reprodutivo da Melipona scutellaris(2025-02-24) Araújo, Nícia Valéria Silva de; Souza, Darclet Teresinha Malerbo de; http://lattes.cnpq.br/3266223126925865; http://lattes.cnpq.br/5123692102259216Este estudo investigou a influência do modelo da caixa e de fatores ambientais na oviposição da abelha sem ferrão Melipona scutellaris. Utilizando um delineamento inteiramente casualizado em arranjo fatorial 2x2, foram comparados dois modelos de caixa (INPA e Nordestino) em condições climáticas seca e chuvosa ao longo de 23 dias, no meliponário pertencente ao Núcleo de Apicultura e Meliponicultura, do Departamento de Zootecnia da Universidade Federal Rural de Pernambuco. As variáveis dependentes incluíram o número de alvéolos fechados, alvéolos abertos e potes de alimento. Análises de variância (ANOVA) indicaram diferenças significativas entre os tratamentos (p<0,05). Testes de Tukey revelaram que o número de alvéolos fechados foi significativamente maior no modelo Nordestino durante o período chuvoso (p = 0,0008), comparado ao modelo INPA e ao período seco. A análise de correlação revelou uma relação positiva entre a umidade relativa do ar e a quantidade de potes de alimento. Esses resultados sugerem que o design da colmeia influencia o microclima interno, afetando diretamente o comportamento de oviposição da rainha. Adicionalmente, as condições climáticas externas, especialmente a umidade, desempenham um papel importante na regulação desses processos. Conclui-se que tanto o tipo da caixa quanto os fatores ambientais são determinantes para o sucesso reprodutivo da M. scutellaris,destacando a importância de considerar essas variáveis no manejo dessas abelhas.Item Meliponini do estado de Pernambuco, Brasil(2021-02-23) Silva, José Augusto dos Santos; Carvalho, Airton Torres; http://lattes.cnpq.br/5995654016063333Meliponini is a tribe of eussocial bees popularly known as stingless bees. This group is recognized for its economic value in the production of honey and other honey products such as wax, pollen and geopropolis. However, it is in the pollination service that these bees stand out. However, it is in the pollination service that these bees stand out. This activity, associated with the ease and practicality of its management, represents an important tool to optimize agricultural productivity and the maintenance of ecosystems. Due to the great demand of the market, there is a need to expand the rational breeding of these animals (meliponiculture), and for this it is necessary to formulate a regulation that guarantees the viability, sustainability and safety of the activity. For the implementation of public policies that supply this demand, one of the requirements is the description of local (state) biodiversity. In this sense, this work is the first checklist of bees from the Meliponini tribe for the state of Pernambuco. The work used two methods to generate a list capable of being used by breeders and decision makers in their actions in favor of species activity and conservation. In the first, metadata from the literature and databases published on the internet (CRIA and GBIF) were compiled and available for consultation (UFMG Collection). In the second part of the work, specimens deposited in the ASA / UFRPE collection belonging to the state were identified using the specific bibliography. In the search for metadata, 34 species were listed, distributed in 14 genera, some records represent dubious determinations, which may be overestimating the actual number of species. In the identification of the material in the collection, 32 species were identified, distributed in 14 genera, some determinations could not be confirmed by the lack of reference material for comparison, and some records deal with exotic species that were translocated from their area of natural occurrence. We list 29 native species occurring in the state of Pernambuco and 3 introduced species. A short diagnosis of each species and photographic plates is made. We present the list and discuss biogeographic and conservation implications.Item Polinização da abóbora (Cucurbita moschata D.): biologia floral, visitantes florais e requerimentos de polinização(2019-07-11) Maciel, Michael dos Santos; Milfont, Marcelo de Oliveira; http://lattes.cnpq.br/0992131397576280; http://lattes.cnpq.br/5349216424195487The objective of this work was to study floral biology, floral visitors and their frequency of visitation, foraging behavior and pollination requirements, among other aspects for the cultivation of pumpkin (Cucurbita moschata D). The experiment was conducted in the city of Garanhuns during the periods of March and June of 2019. An area of 50 m2 was used. The species used was C. moschata D. early Brazilian girl cultivar. Near the plantation were some plants of guava, jamelão, cajá, passion fruit and other herbaceous species in time of flowering. To determine the pollination requirements, the efficiency of free, restricted and cross-pollinated pollination was studied. During 6 days, for 10 min every hour was counted bees that were visiting the flowers. The pollination requirement data were analyzed through analysis of variance through the RStudio program, adopting a significance level of 5%. The foraging frequency data were analyzed using Excel 2013. The flowers of C. moschata D. open at dawn, all being open at 5 o'clock. The stigma was receptive throughout the time the stigma receptivity test was done. The number of male flowers was greater than that of female flowers. The Trigona spinipes was the main visitor in the flowers of the pumpkins, having the peak of visit between 8h and 9h. T. spinipes showed monopoly behavior. Pollen and nectar collection behavior was observed. The female flowers that received the restricted pollination treatment did not produce fruit. The fruiting in the treatment with crossed manual pollination was greater in relation to the open (P <0.05). The ants influenced the least amount of venation in the free pollination, because in large quantities in the female flowers, they prevented the visit of pollinators. The work showed that C. moschata is dependent on pollinators for fruit production; that T. spinipes is the main visitor and possible pollinator of the region and that the ants act as repellents of pollinators, having a negative role in the crop.Item Prospecção de bactérias em colmeia de Melipona mandacaia(2022-10-07) Souza, Maíra Beserra Barbosa de; Melo, Hélio Fernandes de; http://lattes.cnpq.br/6890648212766368; http://lattes.cnpq.br/9288906482369286Melipona mandacaia is a stingless bee species restricted to the Caatinga biome that offers economic and environmental importance. However, biotic and abiotic conditions make bees more susceptible to pathogen attacks. It is known that many microorganisms provide protection to bees and help in the formation of their products. Thus, the objective of this work is to carry out the prospection of bacteria producing organic acids from glucose. Thereby, samples of honey, pollen, geopropolis and trash of bees were collected, stored and taken to the laboratory for analysis. To determine the microbiological quality of samples 1mL of each product was diluted in 9mL of saline (10-1 dilution) and from that, serial dilutions were performed, which were later seeded in Sabouraud agar culture medium (SA) and nutrient agar (NA) being that honey and pollen were sown in both media, but the trash of bees and geopropolis were only sown in NA. The sown microorganisms were placed in the oven and kept for 48 hours at 35ºC for the NA isolates and the SA isolates were placed in the oven for 48 hours at 30ºC. After this procedure, the Colony Forming Units (CFU) were counted. The microorganisms isolated in NA underwent the Red Methyl Test (RM) to verify the production of organic acids. From the RM test, it was verified that the microorganisms isolated from the bee and honey garbage samples produced organic acids from glucose. Therefore, the data obtained revealed that microorganisms isolated from Melipona mandacaia hive products may have antimicrobial activity. Thus, the importance of studying the microbiota associated with stingless bees is emphasized.Item Relatório de Estágio Supervisionado Obrigatório(2023-09-05) Souza, José Felipe Borges de; Souza, Darclet Teresinha Malerbo de; http://lattes.cnpq.br/3266223126925865; http://lattes.cnpq.br/4011173965144301Item Relatório de Estágio Supervisionado Obrigatório(2019-06-05) Rodrigues, Edgleston Silas Ferreira; Souza, Darclet Teresinha Malerbo de; http://lattes.cnpq.br/3266223126925865; http://lattes.cnpq.br/4943460628945352Item Relatório de Estágio Supervisionado Obrigatório(2022-05-26) Fonseca, Tiago Lima de Alcântara; Souza, Darclet Teresinha Malerbo de; http://lattes.cnpq.br/3266223126925865; http://lattes.cnpq.br/1006967947939330Item Relatório de Estágio Supervisionado Obrigatório(2022-10-26) Silva, Thúlio Gustavo da; Souza, Darclet Teresinha Malerbo de; http://lattes.cnpq.br/3266223126925865; http://lattes.cnpq.br/3219343099109016Item Relatório de Estágio Supervisionado Obrigatório(2025-03-17) Santos, Andreilson José dos; Souza, Darclet Teresinha Malerbo de; http://lattes.cnpq.br/3266223126925865Item Relatório de Estágio Supervisionado Obrigatório(2025-03-17) Araújo, Nícia Valéria Silva de; Souza, Darclet Teresinha Malerbo de; http://lattes.cnpq.br/3266223126925865; http://lattes.cnpq.br/5123692102259216Item Relatório de Estágio Supervisionado Obrigatório: atividades desenvolvidas no Setor de Apicultura e Meliponicultura no Departamento de Zootecnia da UFRPE(2022-10-25) Oliveira, Suzykelly Gomes Ferreira de; Pimentel, André Carlos Silva; http://lattes.cnpq.br/7709182422724009Item Relatório do Estágio Supervisionado Obrigatório(2022-10-25) Siqueira, Rodrigo Alves de; Souza, Darclet Teresinha Malerbo de; http://lattes.cnpq.br/3266223126925865; http://lattes.cnpq.br/7596846420584543
